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Root or foliar application
in liquid or water-soluble form

GELAMIN ®: fluid gelatin for industrial use from enzymatic hydrolysis.

GELAMIN ® is derived from an enzymatic hydrolysis production process called FCEH ® (Fully Controlled Enzymatic Hydrolysis) by ILSA.

With a fluid matrix, it is a standardized, stable and homogeneous product, characterized by the predominance of amino acids in the levorotatory form readily absorbable by the plant.

Product suitable for use in organic agriculture, in accordance with Regulations (EU) No. 2018/848 and 2021/1165, NOP Regulation and BR Regulation – Law 10.831/2003). Controlled by ECOCERT SAS.

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operations
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raw material
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and only Gelatin
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Features

Distinctive features

GELAMIN ® It is produced through controlled and highly specific enzymatic hydrolysis which makes it:

  • Highly standardized, pure and homogeneous, and consistent in its Nitrogen content;
  • Contains organic nitrogen completely soluble in water and therefore readily available to plants
  • Rich in intact molecules which are therefore biologically active.
  • Contains a very high number of amino acids, peptides and polypeptides which are fundamental in plant nutrition.
  • The amino acids it contains exert a chelating and complexing function in relation to other elements , reducing the risk of shortages and increasing their mobility.
  • Does not leach when applied at root level and it is rapidly absorbed when applied at leaf level , which means reduced loss.
Discover where GELAMIN® was born
Physicochemical characteristics

Features from GELAMIN ® :

  • high homogeneity;
  • high purity;
  • high stability.

 

The main physical-chemical parameters that characterize the GELAMIN ® are listed in the table.
Table 1. Physicochemical parameters characterizing GELAMIN ® .

Depending on the product based on GELAMIN ® , the amino acid profile does not change, but the percentage of individual amino acids changes based on the specific function of the product.

GELAMIN AMINO ACIDS ® (GLY=GLYCINE; PRO=PROLINE; HYP=HYDROXYPROLINE; GLU=GLUTAMIC ACID; ALA=ALANINE; ARG=ARGININE; ASP=ASPARTIC ACID; SER=SERINE; HIS=HISTIDINE; LYS=LYSINE; LEU=LEUCINE; VAL=VALINE; PHE=PHENYLALANINE; ILE=SOLEUCINE; THR=THREONINE; TYR=TYROSINE; CYS=CYSTEINE; MET=METHIONINE).

Amino acids

During the enzymatic hydrolysis process, protein fractions of variable length are formed (polypeptides, peptides and free amino acids): the molecular size of the peptides can be variable, depending on the intensity of hydrolysis, and ranging from a few hundred to several thousand daltons (Da).

The presence of these fractions with different molecular weights guarantees both the nutritional and biostimulant action of the matrix. GELAMIN ® .

In particular, the biostimulant properties of protein hydrolysates are derived primarily from free amino acids : the amino acid content is therefore an important parameter for the agronomic evaluation of protein hydrolysates.

 

In fact, according to several authors, amino acids, especially in free form, would affect the physiological activities of the plant and in particular :

  • chlorophyll photosynthesis, since glycine is a constituent of chlorophyll;
  • protein synthesis, since glutamic acid, glutamine, aspartic acid and asparagine are the initial amino acids in protein synthesis;
  • a complexing action, since aspartic acid, glutamic acid and glycine are capable of forming chelates with nutrients and molecules of different nature;
  • lignification, since phenylalanine is one of the precursors of lignin biosynthesis;
  • resistance to abiotic stresses, since proline (and hydroxyproline) accumulate in the cytoplasm as a result of water and osmotic stress; alanine (and γ-aminobutyrate) do so in the case of anaerobic stress; glutathione (a compound derived from the amino acid cysteine) do so in the case of the presence of heavy metals and oxidative stress, and; polyamines derived from arginine do so in the case of potassium shortage and water or osmotic stresses;
  • a hormone-like activity, since several amino acids are metabolic precursors of phytohormones (for example, tryptophan is a precursor of indole acetic acid; methionine is of ethylene and; arginine is of polyamines);
  • the fruit ripening processes, since alanine, isoleucine, leucine and valine are precursors of aromas; phenylalanine is a precursor of color, and; alanine, arginine, glycine and proline are precursors of flavor.

Amino acids in gelatin GELAMIN ® are mainly in the L (levorotatory) form, which is biologically active and recognized by plants.
► Levorotatory amino acids maintain their biological activity, since the use of specific enzymes and low temperatures during the enzymatic hydrolysis process allows to reduce the phenomena of racemization of free amino acids, which are typical of chemical or high temperature hydrolysis.

Racemization is a natural phenomenon that leads to the formation of dextrorotatory (in the D form) and levorotatory (in the L form) amino acids from a mixture containing only levorotatory amino acids.
► Racemization occurs very slowly at room temperature, while it is considerably accelerated at high temperatures: this is one of the reasons why dextrorotatory amino acids are virtually absent in nature, and it has been proven that they can also have negative or even toxic effects on living organisms.

Enzymatic hydrolysis

Hydrolysis causes a substance to separate due to the effect of water, and can be

► Chemist : with the help of a chemical substance and therefore with an acidic or basic pH and, in general, at a high temperature.
► Enzymatic : with low temperature (40-50 °C) and pH close to neutral.
► Mixed: chemical + enzymatic.

During the enzymatic hydrolysis process, protein fractions of variable length (polypeptides, peptides and free amino acids) are formed.
Depending on the process adopted, very different protein hydrolysates can be obtained with different agronomic properties.

The main parameters by which the quality of a fluid protein hydrolysate is measured are:

► % total amino acids;
► molecular weight of the various components;
► degree of racemization %: dextrorotatory amino acids / total amino acids.

Amino acids can have two types of structure, levorotatory or dextrorotatory. The L form (levorotatory) is biologically active and recognized by plants.

Compared to chemical hydrolysis, enzymatic hydrolysis allows a high content of levorotatory amino acids (L-form) to be obtained. These amino acids play a relevant role in the plant, they have a biostimulant effect because they act directly on the physiology of the plant. Therefore, protein hydrolysates produced by the enzymatic hydrolysis process are characterized by high quality.

Depending on the method of application, fluid protein hydrolysates obtained by the enzymatic hydrolysis process play diverse roles in plant physiology.

Applying L. amino acids and peptides by foliar application, they are easily absorbed by the leaf apparatus and are therefore directly used by the plant cell in various biochemical processes. In addition, L. amino acids and peptides have a biostimulant action on the plant cell, i.e. they help the plant to overcome abiotic stress (increased temperature, late spring frost, high salinity and drought, etc.) and stimulate the biosynthesis of proteins and DNA.

In addition, L. amino acids stimulate photosynthesis, as well as cell multiplication and expansion, improving vegetative development, fruit swelling and vegetative restart, etc.

Well, using protein hydrolysates by foliar application allows rapid absorption and translocation of amino acids, peptides and polypeptides into the plant. These compounds are organic molecules consisting of organic nitrogen that are rapidly bioavailable to plants.

Applying amino acids and L. peptides by fertigation , protein hydrolysates increase the organic fertility of the soil because they act as an organic substrate for the multiplication of soil microflora.

This improves the physical and chemical conditions of the soil, making nutrients bioavailable to plants. Another benefit is the high percentage of organic nitrogen, which provides high nutritional value to plants.

Furthermore, fluid-protein hydrolysates obtained by enzymatic hydrolysis have the capacity for “complexification” , which allows the absorption and translocation of those elements normally unavailable to plants (Fe, B, Ca, Mg).

The ability to “complex” is due to amino acids in the L-form. Therefore, the higher the content of active amino acids (in the L-form), the greater the ability to provide the plant with microelements.

Ultimately, the fluid-protein hydrolysates obtained by enzymatic hydrolysis have a “coformulating” action. This capacity allows them to be mixed with other products available on the market (fertilizers, commodities, PPPs, PGRs, etc.) improving their effectiveness and absorption by the plant.

Production process

Production process of fluid gelatin for agricultural use.

GELAMIN fluid gelatin ® for agricultural use is produced by means of FULLY CONTROLLED ENZYMATIC HYDROLYSIS FCEH ® .

The process is defined as “mild” because the reaction occurs at low temperatures (around 50-55°C) inside static reactors. The polypeptide chains that form collagen are attacked by a set of specific enzymes, which “cut” peptide bonds in a targeted and replicable manner. Once the hydrolysis stage is complete, vacuum concentration begins…

Discover where GELAMIN® was born

FCEH ® FULLY CONTROLLED ENZYMATIC HYDROLYSIS is an exclusive Ilsa process.

Foliar nutrition

GELAMIN ® is used as a matrix for the formulation of ILSA products used as foliar fertilizers.

Foliar products based on GELAMIN ® are assimilated and translocated within the plant very quickly and, due to the presence of levorotatory amino acids, are readily used in the plant's primary metabolism processes, thus ensuring its uniform and balanced development.

 

Foliar fertilization with products based on GELAMIN ® It also has application benefits, as it ensures rapid absorption, allowing timely action in the event of nutritional deficiencies or critical periods for the plant.

GELAMIN ® is the basis for the formulation of a series of products with nutritional function, applied at the foliar level and containing complexed macro, meso and microelements .

GELAMIN ® in fact, due to its high organic matter content and the presence of free amino acids, peptides and polypeptides, it can form natural complexes or chelates with nutrients, thus increasing its availability for plants and microorganisms.
The action of the organic component and different protein fractions in fluid gelatin GELAMIN ® is then diversified: on the one hand, the complexation and chelation properties help to keep the elements active and usable by plants, even in a wide pH range; in fact, the high presence of amino acids, oligo- and polypeptides whose binding domains are involved in metal-complexation processes, promotes solubility in pH conditions under which, as a rule, the various elements would naturally tend to precipitate .

On the other hand, amino acids and peptides act both as carriers of the elements, facilitating their absorption, and as complexing agents that, by maintaining the elements
in a biologically active form, they favor their translocation and mobility through the phloem transport system. This is the system that carries the substances absorbed by the leaf apparatus to the other organs of the plant (flowers, fruits, seeds, storage organs, roots and young leaves).

In particular, the levorotatory amino acids within the complex GELAMIN ® boron and make it available to fulfill its physiological roles of synthesizing cell wall pectins and transporting complex sugar-borate systems from photosynthetic organs to fruits. As for calcium, an essential component of cell walls, when chelated by amino acids and peptides, it has greater mobility and availability.

Furthermore, the presence of levorotatory amino acids and peptides increases the absorption and translocation of potassium and magnesium in all organs of the plant: the synergy between amino acids, magnesium and potassium allows to improve their activity within the plant.

Microelements such as zinc and manganese , which are important enzyme cofactors, because the amino acids are complexed and more easily transported within the plant, thus allowing rapid and efficient assimilation.

The complexing properties of the matrix GELAMIN ® give products that contain it the property of keeping iron available to the plant and in the biologically active form, that is, the bivalent (Fe 2+ ): it is demonstrated that GELAMIN ® It is designed to maintain iron in solution over a wide pH range in a form that is assimilable by the plant.

So you can conclude that GELAMIN based products ® contain complete elements that provide all the physiological advantages with regard to:

  • favor the transport of zucchini and photoassimilation;
  • increases photosynthetic activity;
  • improving the allegation and formation of new plant tissues;
  • increases tread strength in all conditions rather than increasing fabric consistency;
  • contains isoleucine, istidine, proline and hydroxyproline, which have no structural functions and are not part of the proteins associated with all cell membranes.

Support for plant physiology translates into quantitative and qualitative increases in production in terms of:

  • It is contrasted with the insorgere di carenze;
  • increases the quality and homogeneity of production, thanks to greater color, consistency and fruit taste;
  • increases the durability and conservation of fruits.
Root nutrition

GELAMIN ® It is used as a matrix for the formulation of ILSA products used in fertigation for nutritional purposes.

Its use in fertigation is possible due to its peculiar characteristics, such as:

  • low salinity;
  • high water solubility;
  • perfect miscibility;
  • no bad smells;
  • no phytotoxicity;
  • no leaching;
  • rapid absorption.

 

Products based on GELAMIN ® used in fertigation provide organic nitrogen that is rapidly decomposed by soil bacteria and converted into forms that can be assimilated by the roots (polypeptides, peptides and amino acids), which allows for a rapid response by plants and more homogeneous and balanced vegetative growth.

It was demonstrated, through measuring the increase in soil microbial respiration as a result of its application, that GELAMIN ® stimulates the
population of soil microorganisms.

Furthermore, these products have also been shown to have a direct stimulating effect on root development, which leads to greater root volume and a greater ability to explore the rhizosphere, thus facilitating nutrient uptake.

Agronomic tests confirm that GELAMIN-based products ® guarantee:

  • increased root and leaf biomass;
  • an increase in photosynthetic activity;
  • an increase in production, both in terms of biomass and fruit swelling;
  • an increase in product quality, with a reduction in production waste.
Co-formulation activity

GELAMIN ® It is also the matrix for ILSA products with specific action that can be used in synergy with other fertilizers or products with phytosanitary action (insecticides, fungicides, herbicides, etc.).
Its use in products with a co-formulating function is possible due to its perfect miscibility and solubility.

The characteristics of GELAMIN ® increase the efficiency of use of synergistically distributed products, as GELAMIN ® :

  • increases the wet surface of the sprayed drops, slows down their drying and thus favors the absorption of nutrients;
  • allows, through its carrier function and retention action, the leaf to remain moist for longer due to the ability of the polypeptides to form a semipermeable film that slows down the evaporation of the distributed water;
  • optimizes the action of mixed active substances;
  • transmits the absorption of nutrients, facilitating their access and assimilation via the leaves;
  • promotes the absorption of synthetic organic compounds, thus increasing their effectiveness and consequently reducing their dosage, with benefits in both economic and environmental terms;
  • It does, however, provide proteins, peptides and amino acids with all the functions already widely described.

Its activity as a coformulant is possible due to the presence of amino acids, peptides and polypeptides that can act as carriers, that is, transporters of other molecules across the leaf surface.

The positive influence of GELAMIN ® in the transport of pesticides is visible especially in the absorption, translocation and toxicity of the active ingredient, since the effectiveness of the latter is increased: protein hydrolysates in fact contain amino acids and peptides that are able to actively overcome cell membranes and act in
different metabolic pathways. Therefore, the penetration and translocation of other molecules are facilitated, both directly through active transport mechanisms (symport) and indirectly by increasing the free energy available to the cell for active transport mechanisms.

The positive influence of the matrix on fertilizer use efficiency is due to its ability to increase the retention of aqueous solutions containing fertilizers on the leaf edges. In addition, the product, as a result of increased retention, also increases the surface area between the droplets and the leaf blade, thereby increasing the absorption of the applied nutrient solution.

From an agronomic point of view, the application of products containing GELAMIN ® has several positive effects on the plant's physiology, such as:

  • acceleration of the main metabolisms and absorption of nutrients, since loss through foliar leaching decreases and potentially absorbable quantities increase;
  • an increase in production and an improvement in product quality;
  • an increase in the ability to overcome phases of stress;
  • increased leaf and root biomass;
  • an improvement in quantitative and qualitative production parameters;
  • a strong buffering effect capable of reducing the negative effects caused by extreme changes in salinity.
Stimulating action

GELAMIN ® is used as a matrix to formulate products ILSA used as foliar fertilizers. It has been shown that its characteristics allow it to be used both as a nutritional and as a stimulant: the stimulating activity depends mainly on some amino acids which, through the process of enzymatic hydrolysis at low temperatures, remain intact.

In the composition of the GELAMIN ® , in fact, in addition to amino acids involved in primary metabolisms (protein synthesis, photosynthesis…), there are also amino acids with a biological function in secondary metabolisms that affect resistance to abiotic stress on the one hand, while improving production quality on the other.

A clear plant stimulation activity was widely demonstrated, both in the leaf and root apparatus, with an increase in leaf and root masses without any real increase in nitrogen nutritional levels.

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